In this study we suggest a combined approach for the sperm analysis in which DH and RS work together, providing complementary, label-free, non-destructive qualitative and quantitative information on bovine spermatozoa. DH and RS allows high resolution morphological analysis and chemical assessment of the highlighted morphological abnormalities, respectively. The same combined-approach has also been tested as assessing method for identifying X- and Y-bearing sperm cells, in order to show their suitability to control and preselect the sex of breeding animal offspring.
A combined holographic and raman microscopy approach for the assessment of spermatozoa
De Angelis A;Ferrara M A;Sirleto L;Coppola G;De Luca A C
2015
Abstract
In this study we suggest a combined approach for the sperm analysis in which DH and RS work together, providing complementary, label-free, non-destructive qualitative and quantitative information on bovine spermatozoa. DH and RS allows high resolution morphological analysis and chemical assessment of the highlighted morphological abnormalities, respectively. The same combined-approach has also been tested as assessing method for identifying X- and Y-bearing sperm cells, in order to show their suitability to control and preselect the sex of breeding animal offspring.File in questo prodotto:
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