Nowadays bioengineering and biomedical research are mainly focused on the development of new technologies to improve diagnosis of complex diseases. Nanotechnology allows the design and manipulation of materials in nanoscale at very low cost, to be utilized in different fields such as clinic diagnosis. Recently, it has been demonstrated that ferritin based nanoparticles (Ft-NPs) are an exceptionally versatile tool both for diagnosis and drug delivery. In this work we will develop Ft-NPs as nanodevice for improving the detection, sensitivity, speed and accuracy of biomarkers to utilize in clinical tumor diagnosis. By different techniques as immunofluorescence, cytofluorometry, Western blot, we have analyzed the different expression of some biomarkers in different tumor cell lines. In particular we analyzed the level of expression of TrkB Receptor to utilize as neuroblastoma prognostic factor; EP2 and EP4 receptors (for PGE2) to utilize as prognostic factors for lung adenocarcinoma; ICAM-1 and FASL to utilize as lung carcinoma and mesothelioma prognostic factors. On the basis of these results we have planed to functionalize Ft-NPs with the ligands specific for these tumor biomarkers to be employed as diagnostic tools. The research was supported by MIUR, Flagship Project NanoMAX.
Functionalized nanoparticles with ligands of tumor biomarkers for a diagnostic approach
P Picone;M Ferraro;M Montalbano;E Messina;L Siena;E Pace;M Profita;M Di Carlo
2015
Abstract
Nowadays bioengineering and biomedical research are mainly focused on the development of new technologies to improve diagnosis of complex diseases. Nanotechnology allows the design and manipulation of materials in nanoscale at very low cost, to be utilized in different fields such as clinic diagnosis. Recently, it has been demonstrated that ferritin based nanoparticles (Ft-NPs) are an exceptionally versatile tool both for diagnosis and drug delivery. In this work we will develop Ft-NPs as nanodevice for improving the detection, sensitivity, speed and accuracy of biomarkers to utilize in clinical tumor diagnosis. By different techniques as immunofluorescence, cytofluorometry, Western blot, we have analyzed the different expression of some biomarkers in different tumor cell lines. In particular we analyzed the level of expression of TrkB Receptor to utilize as neuroblastoma prognostic factor; EP2 and EP4 receptors (for PGE2) to utilize as prognostic factors for lung adenocarcinoma; ICAM-1 and FASL to utilize as lung carcinoma and mesothelioma prognostic factors. On the basis of these results we have planed to functionalize Ft-NPs with the ligands specific for these tumor biomarkers to be employed as diagnostic tools. The research was supported by MIUR, Flagship Project NanoMAX.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
