High molecular weighl glulenin subunits present in several accessions of tetraploid wheats with genomic formula AAGG, T.tlimopheevii (culltvated) and subsp. araraticum (wild), were analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed phase high performance liquid chromatography (RP-HPLC), Combination of these techniques permitted us to associate different type of subunits to the A or the G genome and assess the extent of allelic variation present at corresponding encoding loci. Results also revealed that in both subspecies accessions are present possessing expressed ytype subunits at the Glu-A 1 locus. This is in contrast with what was observed in tetraploid wheats of genomic formula AABB, where only in the wild species T. turgidum subsp. dicoccoides such subunit is expressed. Electrophoretic and chromatographic behaviour of x-and y-type subunits, present al the Glu-A 1 locus in both subspecies, is very similar lo the one observed in the wild diploid wheat relative T. urartu. This result supports the raie of this species in the formation of polyploid wheals of the timopheevii group.
Variation of high molecular weight glutenin subunits in tetraploid wheat T.thimopheevi
Margiotta B;Urbano M;Colaprico G;
1997
Abstract
High molecular weighl glulenin subunits present in several accessions of tetraploid wheats with genomic formula AAGG, T.tlimopheevii (culltvated) and subsp. araraticum (wild), were analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed phase high performance liquid chromatography (RP-HPLC), Combination of these techniques permitted us to associate different type of subunits to the A or the G genome and assess the extent of allelic variation present at corresponding encoding loci. Results also revealed that in both subspecies accessions are present possessing expressed ytype subunits at the Glu-A 1 locus. This is in contrast with what was observed in tetraploid wheats of genomic formula AABB, where only in the wild species T. turgidum subsp. dicoccoides such subunit is expressed. Electrophoretic and chromatographic behaviour of x-and y-type subunits, present al the Glu-A 1 locus in both subspecies, is very similar lo the one observed in the wild diploid wheat relative T. urartu. This result supports the raie of this species in the formation of polyploid wheals of the timopheevii group.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
