Glutenin polymers are formed by high (HMW-GS) and low molecular weigbt glutenin subunits (LMW-GS). The latter group of subunits bave been less characterised compared to tbe former due to their great number and heterogeneity. LMW-GS have been subdivided into B, C and D groups on the basis of their biochemical differences, though only subunits included in the B group are considered typical LMW-GS. In fact, it has been demonstrated that C and D groups correspond to mutated gliadins in which a different organisation of their cysteine residues has enable them to form intermolecular disulphide bonds. Typical LMW-GS have been classified into three major groups, namely LMW-s, LMW-m and LMW-i types according to their first amino acid residue. The latter group of subunits has been found to have a few peculiarities compared to the first two, such as a different distribution of cysteine residues (which could affect capability of these subunits in glutenin polymer formation and dough quality characteristic) and encoding genes associated only to the chromosome 1A. In order to gain more information on the LMW-GS we bave used a durum wheat line carrying a 1BL. 1RS translocation, in which the short arm of the chromosome 1B is replaced by the short arm of the chromosome 1R of rye. This line was obtained using the durum wheat cultivar Cando, in which the transiocation is present, crossed and back-crossed three times with the italian durum wheat cultivar Svevo. Comparative electrophoretic, chromatographic and mass spectrometry analyses carried out on LMW-GS prepared from the durum wheat cultivar Svevo and line canying the 1BL. 1 RS translocation bave provided further information on these complex group of proteins.
Characterisation of B- and C-type Low Molecular Weight Glutenin Subunits in Durum Wheat
M URBAN;G COLAPRIC;
2006
Abstract
Glutenin polymers are formed by high (HMW-GS) and low molecular weigbt glutenin subunits (LMW-GS). The latter group of subunits bave been less characterised compared to tbe former due to their great number and heterogeneity. LMW-GS have been subdivided into B, C and D groups on the basis of their biochemical differences, though only subunits included in the B group are considered typical LMW-GS. In fact, it has been demonstrated that C and D groups correspond to mutated gliadins in which a different organisation of their cysteine residues has enable them to form intermolecular disulphide bonds. Typical LMW-GS have been classified into three major groups, namely LMW-s, LMW-m and LMW-i types according to their first amino acid residue. The latter group of subunits has been found to have a few peculiarities compared to the first two, such as a different distribution of cysteine residues (which could affect capability of these subunits in glutenin polymer formation and dough quality characteristic) and encoding genes associated only to the chromosome 1A. In order to gain more information on the LMW-GS we bave used a durum wheat line carrying a 1BL. 1RS translocation, in which the short arm of the chromosome 1B is replaced by the short arm of the chromosome 1R of rye. This line was obtained using the durum wheat cultivar Cando, in which the transiocation is present, crossed and back-crossed three times with the italian durum wheat cultivar Svevo. Comparative electrophoretic, chromatographic and mass spectrometry analyses carried out on LMW-GS prepared from the durum wheat cultivar Svevo and line canying the 1BL. 1 RS translocation bave provided further information on these complex group of proteins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
