The endoplasmic reticulum takes care of the folding, assembly and quality control of thousands of proteins destined to the different compartments of the endomembrane system, or to be secreted in the apoplast. Here we describe how these early events in the life of all these proteins can be followed biochemically by using velocity or isopycnic ultracentrifugation, metabolic labelling with radioactive amino acids and immunoprecipitation in various conditions.
"Protein Biosynthesis and Maturation in the ER"
Pedrazzini Emanuela;Vitale Alessandro
2018
Abstract
The endoplasmic reticulum takes care of the folding, assembly and quality control of thousands of proteins destined to the different compartments of the endomembrane system, or to be secreted in the apoplast. Here we describe how these early events in the life of all these proteins can be followed biochemically by using velocity or isopycnic ultracentrifugation, metabolic labelling with radioactive amino acids and immunoprecipitation in various conditions.File in questo prodotto:
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