A procedure for a spatially selective immobilization of antibodies, via photochemical activation, on the inner surface of optical microbubble resonators (OMBRs) is reported. The method is based on a suitable UV mask process. As proof of concept of this technique, immunoassay in photo-activated OMBR was implemented and fluorescence investigation was conducted. Moreover, the possible application of this procedure towards the development of a biochemical multiplexed detection system based on OMBR is presented. Finally, the high Q factor values measured after the immunoassay event (>105) make these photo-activated OMBRs suitable devices for future label-free biosensor implementation.
Fluorescence biosensing in selectively photo-activated microbubble resonators
Simone Berneschi;Francesco Baldini;Alessandro Cosci;Daniele Farnesi;Gualtiero Nunzi Conti;Sara Tombelli;Cosimo Trono;Stefano Pelli;Ambra Giannetti
2017
Abstract
A procedure for a spatially selective immobilization of antibodies, via photochemical activation, on the inner surface of optical microbubble resonators (OMBRs) is reported. The method is based on a suitable UV mask process. As proof of concept of this technique, immunoassay in photo-activated OMBR was implemented and fluorescence investigation was conducted. Moreover, the possible application of this procedure towards the development of a biochemical multiplexed detection system based on OMBR is presented. Finally, the high Q factor values measured after the immunoassay event (>105) make these photo-activated OMBRs suitable devices for future label-free biosensor implementation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
