In this work, an approach based on digital holography (DH) combined with Raman spectroscopy (RS) is used for a complete label-free analysis of single bovine spermatozoa. DH allows to obtain high-resolution images of bovine sperm from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Raman imaging analysis have also confirmed such anomalies, suggesting the protein vibrations as associated Raman marker of the defect.
Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach
De Luca AC
2015
Abstract
In this work, an approach based on digital holography (DH) combined with Raman spectroscopy (RS) is used for a complete label-free analysis of single bovine spermatozoa. DH allows to obtain high-resolution images of bovine sperm from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Raman imaging analysis have also confirmed such anomalies, suggesting the protein vibrations as associated Raman marker of the defect.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
