We report the synthesis and characterization of two oligonucleotides (a tetramer and a 19-mer) labelled with a fluorescent oligothiophene and obtained by means of the phosphoramidite of the fluorophore. The conjugate compounds were synthesized in solid phase and characterized by means of mass spectrometry, multinuclear NMR, UV-vis and photoluminescence spectroscopies. The results show that this approach is suitable as a general route for the preparation of oligonucleotides labelled with oligothiophene-based fluorophores. (c) 2005 Published by Elsevier Ltd.
Oligothiophene phosphoramidites for oligonucleotide labelling.
M Zambianchi;G Barbarella
2005
Abstract
We report the synthesis and characterization of two oligonucleotides (a tetramer and a 19-mer) labelled with a fluorescent oligothiophene and obtained by means of the phosphoramidite of the fluorophore. The conjugate compounds were synthesized in solid phase and characterized by means of mass spectrometry, multinuclear NMR, UV-vis and photoluminescence spectroscopies. The results show that this approach is suitable as a general route for the preparation of oligonucleotides labelled with oligothiophene-based fluorophores. (c) 2005 Published by Elsevier Ltd.File in questo prodotto:
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