Colony morphology (CM) is an important criterion to evaluate health of Pluripotent Stem Cells (PSCs) in culture or to select induced-PSC colonies after reprogramming. However, manual evaluation of CM is time-consuming, not quantitative and poorly reproducible. We designed an unbiased method to evaluate CM of non-labeled PSCs using microplate images acquired from a flatbed scanner. The high-throughput automated analysis is based on image processing and algorithms for segmentation, count and multi-parametric classification of colonies.
Imaging for High-Throughput Screening of Pluripotent Stem Cells
L Casalino;M R Guarracino;L Maddalena
2018
Abstract
Colony morphology (CM) is an important criterion to evaluate health of Pluripotent Stem Cells (PSCs) in culture or to select induced-PSC colonies after reprogramming. However, manual evaluation of CM is time-consuming, not quantitative and poorly reproducible. We designed an unbiased method to evaluate CM of non-labeled PSCs using microplate images acquired from a flatbed scanner. The high-throughput automated analysis is based on image processing and algorithms for segmentation, count and multi-parametric classification of colonies.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
