Bone and dentin are nano-structured composites containing mineralized collagenous tissues. Composite scaffolds consisting in biodegradable natural polymers are one of the key components of bone tissue-engineering strategies. It has been reported that hydroxyapatite (HAp) can support alginate (Alg) as inorganic and osteoconductive reinforcement of Alg/HAp-based composite scaffolds. The first aim of our study was to evaluate the biocompatibility and the regenerative potential of these novel constructs in a dental pulp mesenchymal stem cells (DPSCs) - 3D in vitro system in the presence of differentiation medium (DM). There is evidence that osteointegration failure and inflammation are frequent complications of implantology. Interleukin-6 (IL-6) acts in several in vivo cell processes such as immune regulation and tissue regeneration, and plays a pivotal role during mesenchymal stem cell commitment to the osteogenic lineage. Moreover the maintenance of a redox homeostasis seems to be crucial during stem cell differentiation. In this light, we afterwards analyzed the molecular pathways underlying the intracellular balance between redox homeostasis and extracellular matrix mineralization.
Alginate/hydroxyapatite composite scaffolds for bone ingrowth: a biocompatibility and molecular study.
Monica Rapino;
2020
Abstract
Bone and dentin are nano-structured composites containing mineralized collagenous tissues. Composite scaffolds consisting in biodegradable natural polymers are one of the key components of bone tissue-engineering strategies. It has been reported that hydroxyapatite (HAp) can support alginate (Alg) as inorganic and osteoconductive reinforcement of Alg/HAp-based composite scaffolds. The first aim of our study was to evaluate the biocompatibility and the regenerative potential of these novel constructs in a dental pulp mesenchymal stem cells (DPSCs) - 3D in vitro system in the presence of differentiation medium (DM). There is evidence that osteointegration failure and inflammation are frequent complications of implantology. Interleukin-6 (IL-6) acts in several in vivo cell processes such as immune regulation and tissue regeneration, and plays a pivotal role during mesenchymal stem cell commitment to the osteogenic lineage. Moreover the maintenance of a redox homeostasis seems to be crucial during stem cell differentiation. In this light, we afterwards analyzed the molecular pathways underlying the intracellular balance between redox homeostasis and extracellular matrix mineralization.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
