The combination of a microscope and a Raman spectrometer provides insights to a multi-stepped surface modification process to fabricate a biosensor for avidin detection using biotin-modified, gold-coated silicon nanowires (Au/SiNWs) deposited on a glass substrate. It is demonstrated that the silicon nanowires morphology enhances light absorption in the overlaying Au layer. In addition, the Raman spectra unequivocally confirmed that the composite structure (SiNWs + Au + Cysteamine + Biotin) can effectively bind the target molecule avidin through specific receptor-ligand interactions
Using Raman microscopy to monitor the surface modifications of disordered array of gold (Au) covered silicon nanowires for SERS biosensing
Valentina Mussi;Annalisa Convertino;Luca Maiolo;
2018
Abstract
The combination of a microscope and a Raman spectrometer provides insights to a multi-stepped surface modification process to fabricate a biosensor for avidin detection using biotin-modified, gold-coated silicon nanowires (Au/SiNWs) deposited on a glass substrate. It is demonstrated that the silicon nanowires morphology enhances light absorption in the overlaying Au layer. In addition, the Raman spectra unequivocally confirmed that the composite structure (SiNWs + Au + Cysteamine + Biotin) can effectively bind the target molecule avidin through specific receptor-ligand interactionsFile in questo prodotto:
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