An efficient method has been developed to improve preparation of phage particles by ammonium sulfate precipitation and to yield high quality DNA. The method, that has been used to screen plant DNA libraries constructed in lambda vectors, is inexpensive, does not require purification of phage particles, and can be used from either plate stocks or liquid lysates. Up to 1100 micrograms DNA was produced from 5 ml lysate obtained from agar plates.
An efficient method for preparing high quality DNA from plant DNA libraries in lambda phage
Del Giudice L
2002
Abstract
An efficient method has been developed to improve preparation of phage particles by ammonium sulfate precipitation and to yield high quality DNA. The method, that has been used to screen plant DNA libraries constructed in lambda vectors, is inexpensive, does not require purification of phage particles, and can be used from either plate stocks or liquid lysates. Up to 1100 micrograms DNA was produced from 5 ml lysate obtained from agar plates.File in questo prodotto:
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