The detection of the mixed microbial communities in an environmental sample has represented a hurdle for long time due to the selection imposed by the culturing on specific media and by the minimum number of cells required. Here, we simulated a contamination of a sterile radiochemical by adding a known amount of Staphylococcus epidermidis and we developed a methodology, based on a metagenomic approach, to isolate DNA without a previous selection, from a low starting material and in limited time. The obtained sequences have been further used to characterize the microbial populations present in the sample using a bioinformatics tool.
Metagenomic characterization of the entire microbial community in a radiochemical sample
Elda Perlino
2018
Abstract
The detection of the mixed microbial communities in an environmental sample has represented a hurdle for long time due to the selection imposed by the culturing on specific media and by the minimum number of cells required. Here, we simulated a contamination of a sterile radiochemical by adding a known amount of Staphylococcus epidermidis and we developed a methodology, based on a metagenomic approach, to isolate DNA without a previous selection, from a low starting material and in limited time. The obtained sequences have been further used to characterize the microbial populations present in the sample using a bioinformatics tool.File in questo prodotto:
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