Specimens of Nectria spp. and Nectriella rufofusca were obtained from the fungarium of Pier AndreaSaccardo, and investigated via a morphological and molecular approach based on MiSeq technology. ITS1 andITS2 sequences were successfully obtained from 24 specimens identified as 'Nectria' sensu Saccardo (including20 types) and from the type specimen of Nectriella rufofusca. For Nectria ambigua, N. radians and N. tjibodensisonly the ITS1 sequence was recovered. On the basis of morphological and molecular analyses new nomenclaturalcombinations for Nectria albofimbriata, N. ambigua, N. ambigua var. pallens, N. granuligera, N. peziza subsp.reyesiana, N. radians, N. squamuligera, N. tjibodensis and new synonymies for N. congesta, N. flageoletiana,N. phyllostachydis, N. sordescens and N. tjibodensis var. crebrior are proposed. Furthermore, the current classificationisconfirmedforNectriacoronata,N.cyanostoma,N.dolichospora,N.illudens,N.leucotricha,N.mantuana,N.raripilaand Nectriellarufofusca.Thisisthefirsttimethatthesemorethan100-yr-oldspecimensaresubjectedtomolecular analysis, thereby providing important new DNAsequence data authentic for these names.
Illuminating type collections of nectriaceous fungi in Saccardo's fungarium
Vizzini A;Voyron S;Girlanda M;
2020
Abstract
Specimens of Nectria spp. and Nectriella rufofusca were obtained from the fungarium of Pier AndreaSaccardo, and investigated via a morphological and molecular approach based on MiSeq technology. ITS1 andITS2 sequences were successfully obtained from 24 specimens identified as 'Nectria' sensu Saccardo (including20 types) and from the type specimen of Nectriella rufofusca. For Nectria ambigua, N. radians and N. tjibodensisonly the ITS1 sequence was recovered. On the basis of morphological and molecular analyses new nomenclaturalcombinations for Nectria albofimbriata, N. ambigua, N. ambigua var. pallens, N. granuligera, N. peziza subsp.reyesiana, N. radians, N. squamuligera, N. tjibodensis and new synonymies for N. congesta, N. flageoletiana,N. phyllostachydis, N. sordescens and N. tjibodensis var. crebrior are proposed. Furthermore, the current classificationisconfirmedforNectriacoronata,N.cyanostoma,N.dolichospora,N.illudens,N.leucotricha,N.mantuana,N.raripilaand Nectriellarufofusca.Thisisthefirsttimethatthesemorethan100-yr-oldspecimensaresubjectedtomolecular analysis, thereby providing important new DNAsequence data authentic for these names.| File | Dimensione | Formato | |
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Descrizione: Forin et al PR 2020
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