The material used in bone regeneration need to be compatible, osteoconductive and osteointegrative and to have mechanical strenght to provide a structural support. Among biodegradable natural polymers, hydroxyapatite (HAp) can support alginate as inorganic reinforcement and osteoconductive component. In the present experimental system, Hap was integrated in an alginate solution and treated to obtain a direct release of calcium ions. The resulting alginate/HAp composite scaffolds are very promising constructs enable to support bone regeneration. Human Dental Pulp Stem Cells (DPSCs) can be easily isolated from the surgical waste derived from deciduous teeth or wisdom teeth extraction and can differentiate into various cell types, namely cells belonging to osteogenic, dentinogenic, adipogenic, neurogenic, chondrogenic and myogenic lineage. Aim of the present study is to verify the mineralization and differentiation potential of human DPSCs seede onto scaffolds based on alginate and nano-hydroxyapatite. Viability parameters, oxidative stress occurrence and catalase activity, gene expression profile of early and late mineralization-related markers, and the expression and secretion of extracellular matrix components were evaluated and analyzed. Our results demonstrated that DPSCs express osteogenic differentiation-related markers and enhance calcium deposition and biomineralization when growth onto Alg/HAp scaffolds. Most important, this physiological function is here related to redox homeostasis controlled by the activation of catalase which, as an enzymatic antioxidant, enhances cell survival. The osteoconductivity of the alginate/hydroxyapatite scaffolds was assessed and the scaffolds were found able to efficiently sustain adhesion, colonization and matrix deposition of osteoblast-like cells.
Alginate/Hydroxyapatite scaffolds promote adhesion, colonization and matrix deposition of osteoblast-like cells differentiated from dental pulp stem cells
M Rapino;
2018
Abstract
The material used in bone regeneration need to be compatible, osteoconductive and osteointegrative and to have mechanical strenght to provide a structural support. Among biodegradable natural polymers, hydroxyapatite (HAp) can support alginate as inorganic reinforcement and osteoconductive component. In the present experimental system, Hap was integrated in an alginate solution and treated to obtain a direct release of calcium ions. The resulting alginate/HAp composite scaffolds are very promising constructs enable to support bone regeneration. Human Dental Pulp Stem Cells (DPSCs) can be easily isolated from the surgical waste derived from deciduous teeth or wisdom teeth extraction and can differentiate into various cell types, namely cells belonging to osteogenic, dentinogenic, adipogenic, neurogenic, chondrogenic and myogenic lineage. Aim of the present study is to verify the mineralization and differentiation potential of human DPSCs seede onto scaffolds based on alginate and nano-hydroxyapatite. Viability parameters, oxidative stress occurrence and catalase activity, gene expression profile of early and late mineralization-related markers, and the expression and secretion of extracellular matrix components were evaluated and analyzed. Our results demonstrated that DPSCs express osteogenic differentiation-related markers and enhance calcium deposition and biomineralization when growth onto Alg/HAp scaffolds. Most important, this physiological function is here related to redox homeostasis controlled by the activation of catalase which, as an enzymatic antioxidant, enhances cell survival. The osteoconductivity of the alginate/hydroxyapatite scaffolds was assessed and the scaffolds were found able to efficiently sustain adhesion, colonization and matrix deposition of osteoblast-like cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
