Chitlac-coated BisGMA/TEGDMA methacrylic thermosets are a very versatile new kind of biomaterial for both dentistry and orthopaedic applications. The coating with a lactose-modified chitosan make them a "bioactive" biomaterial, able to induce specific biological responses by engaging interactions with the surrounding living tissue at the molecular level. Chitlac thermosets do to not affect viability and proliferation of different cell lines as well as primary cells. When the Chitlac coating is enriched with silver nanoparticles (nAG), an important antibacterial activity was demonstrated, but their most important feature is the ability to enhance chondrogenic differentiation and bone formation, as demonstrated also by in vivo tests. Dental pulp stem cells (DPSCs) represent a population of stem cells that are able to differentiate into mature odontoblasts/osteoblasts that produce mineralized matrix. They are part of a periendothelial niche, in which their crosstalk with endothelial cells is important in the cellular response to biomaterilas used in restorative practice. Endothelial cells are therefore equally as important in bone regeneration, given their role in producing modulators of bone development, maintaining vascular homeostasis and secreting molecules which control cell proliferation. DPSCs were co-cultured with the endothelial cell line EA.hy926 in presence of Chitlac-coated BisGMA/TEGDMA thermosets, whose effects on cellular osteogenic and angiogenic potential were evaluated.
Chitlac-coated methacrylic thermosets improve the osteo- and angiogenic potential of a co-culture of dental pulp stem cells and endothelial cells.
Monica Rapino;
2019
Abstract
Chitlac-coated BisGMA/TEGDMA methacrylic thermosets are a very versatile new kind of biomaterial for both dentistry and orthopaedic applications. The coating with a lactose-modified chitosan make them a "bioactive" biomaterial, able to induce specific biological responses by engaging interactions with the surrounding living tissue at the molecular level. Chitlac thermosets do to not affect viability and proliferation of different cell lines as well as primary cells. When the Chitlac coating is enriched with silver nanoparticles (nAG), an important antibacterial activity was demonstrated, but their most important feature is the ability to enhance chondrogenic differentiation and bone formation, as demonstrated also by in vivo tests. Dental pulp stem cells (DPSCs) represent a population of stem cells that are able to differentiate into mature odontoblasts/osteoblasts that produce mineralized matrix. They are part of a periendothelial niche, in which their crosstalk with endothelial cells is important in the cellular response to biomaterilas used in restorative practice. Endothelial cells are therefore equally as important in bone regeneration, given their role in producing modulators of bone development, maintaining vascular homeostasis and secreting molecules which control cell proliferation. DPSCs were co-cultured with the endothelial cell line EA.hy926 in presence of Chitlac-coated BisGMA/TEGDMA thermosets, whose effects on cellular osteogenic and angiogenic potential were evaluated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
