The healing properties of plants have always been well known and many recent studies are focused on the neuroprotection exerted by various species in different experimental models. In the present study, the biological effects of water extracts of Salix Alba and Parthenium were investigated in two different models: in vitro, exposing CTX-TNA2 astrocyte cells to hydrogen peroxide and ex vivo, treating rat cortexes with a neurotoxic concentration of potassium, mimicking the cortical spreading depression (CSD). MTT assay was performed and LDH release was quantified to investigate cytotoxicity, apoptosis occurrence was evaluated by Annexin-V/PI flow cytometry assay, while nitrite levels and 5HTA/5HT ratio were measured as indexes of cortex functionality. Different concentrations of both extracts were screened by MTT assay (100-200 ug/ml for Salix and 60-120 ug/ml for Parthenium) and one concentration for each plant extract was chosen for further analyses. Both S. alba and Parthenium proved to be able to counteract the cytotoxicity and the apoptosis occurrence triggered by the treatment of CTX-TNA2 cells with hydrogen peroxide. As for the cortex samples, the exposure to the concentration of 60 mM of potassium, mimicking the CDS, increased LDH release, nitrite levels and 5HTA/5HT ratio. The administration of the extracts of both plants restore all the parameters to values similar to the control samples, with a greater effect found in the cortexes exposed to the Salix alba. Concluding, our data, demonstrating the protective effect exterted by Salix alba and Parthenium extracts on both CTX-TNA2 astrocyte cells and isolated rat cortexes, suggest potential applications for these extracts in the tratment of clinical symptoms related to neuroinflammation.
Protective effects of Salix alba and Parthenium extracts in two experimental models of neurotoxicity.
Monica Rapino;
2019
Abstract
The healing properties of plants have always been well known and many recent studies are focused on the neuroprotection exerted by various species in different experimental models. In the present study, the biological effects of water extracts of Salix Alba and Parthenium were investigated in two different models: in vitro, exposing CTX-TNA2 astrocyte cells to hydrogen peroxide and ex vivo, treating rat cortexes with a neurotoxic concentration of potassium, mimicking the cortical spreading depression (CSD). MTT assay was performed and LDH release was quantified to investigate cytotoxicity, apoptosis occurrence was evaluated by Annexin-V/PI flow cytometry assay, while nitrite levels and 5HTA/5HT ratio were measured as indexes of cortex functionality. Different concentrations of both extracts were screened by MTT assay (100-200 ug/ml for Salix and 60-120 ug/ml for Parthenium) and one concentration for each plant extract was chosen for further analyses. Both S. alba and Parthenium proved to be able to counteract the cytotoxicity and the apoptosis occurrence triggered by the treatment of CTX-TNA2 cells with hydrogen peroxide. As for the cortex samples, the exposure to the concentration of 60 mM of potassium, mimicking the CDS, increased LDH release, nitrite levels and 5HTA/5HT ratio. The administration of the extracts of both plants restore all the parameters to values similar to the control samples, with a greater effect found in the cortexes exposed to the Salix alba. Concluding, our data, demonstrating the protective effect exterted by Salix alba and Parthenium extracts on both CTX-TNA2 astrocyte cells and isolated rat cortexes, suggest potential applications for these extracts in the tratment of clinical symptoms related to neuroinflammation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
