Stimulated Raman scattering (SRS) microscopy allows vibrational contrast mechanism for imaging with high spectral and spatial resolution along with three-dimensional sectioning. In this paper, taking advantage of a SRS vibrational contrast, a fully 3D volume reconstruction of lipids droplets distribution in adipocyte cells is demonstrated.
3D reconstruction of lipid droplets in single cells by stimulated raman imaging
Ferrara MA;Ranjan R;Filograna A;D'Arco A;Valente C;Larobina M;Sirleto L
2018
Abstract
Stimulated Raman scattering (SRS) microscopy allows vibrational contrast mechanism for imaging with high spectral and spatial resolution along with three-dimensional sectioning. In this paper, taking advantage of a SRS vibrational contrast, a fully 3D volume reconstruction of lipids droplets distribution in adipocyte cells is demonstrated.File in questo prodotto:
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