Legume species are a major source of protein for both human and animal nutrition. Proteins from legumes possess the highest sustainability because, among other reasons, legume plants have positive impact on soil fertility due to their ability to fix nitrogen via rhizobial symbioses. Medicago truncatula was chosen as a model for legumes because it is diploid, with a relatively small genome (450-550 Mb), self-fertile, relatively easy to transform, and it has a short generation time. In the last years several mutant collections became available in both Europe and USA, based on different strategies: chemical mutagenesis (TILLING); deletion mutagenesis (fast-neutron bombardment and -rays); insertional mutagenesis (transposons such as Tnt1) (Porceddu et al, 2008; Tadege et al., 2009, Rogers et al., 2009 ). Part of these collections were produced in Italy and used for isolating mutants of interest related to traits of practical relevance, in particular a key gene involved in saponin biosynthesis was identified. The possibility to speed up knowledge of the genetic control of traits of agronomic interest and translate such knowledge to crop legumes via different means will be discussed. Porceddu A, Panara F, Calderini O, Molinari L, Taviani P, Lanfaloni L, Scotti C, Carelli M, Scaramelli L, Bruschi G, Cosson V, Ratet P, de Larembergue H, Duc G, Piano E, Arcioni S (2008) An Italian functional genomic resource for Medicago truncatula BMC Res Notes 1:129. Rogers C, Wen J, Chen R, Oldroyd G Deletion-based reverse genetics in Medicago truncatula Plant Physiol. 2009 151(3):1077-86. Tadege M, Wang TL,Wen J, Ratet P, Mysore KS (2009) Mutagenesis and beyond! Tools for understanding legume biology. Plant Physiol. 151(3):978-84.
FUNCTIONAL GENOMICS OF MEDICAGO TRUNCATULA, A MODEL FOR LEGUME SPECIES: RESULTS AND PROSPECTS OF TRANSLATION TO CROPS.
PAOLOCCI F;PASSERI V;DAMIANI F;ARCIONI S;CALDERINI O
2010
Abstract
Legume species are a major source of protein for both human and animal nutrition. Proteins from legumes possess the highest sustainability because, among other reasons, legume plants have positive impact on soil fertility due to their ability to fix nitrogen via rhizobial symbioses. Medicago truncatula was chosen as a model for legumes because it is diploid, with a relatively small genome (450-550 Mb), self-fertile, relatively easy to transform, and it has a short generation time. In the last years several mutant collections became available in both Europe and USA, based on different strategies: chemical mutagenesis (TILLING); deletion mutagenesis (fast-neutron bombardment and -rays); insertional mutagenesis (transposons such as Tnt1) (Porceddu et al, 2008; Tadege et al., 2009, Rogers et al., 2009 ). Part of these collections were produced in Italy and used for isolating mutants of interest related to traits of practical relevance, in particular a key gene involved in saponin biosynthesis was identified. The possibility to speed up knowledge of the genetic control of traits of agronomic interest and translate such knowledge to crop legumes via different means will be discussed. Porceddu A, Panara F, Calderini O, Molinari L, Taviani P, Lanfaloni L, Scotti C, Carelli M, Scaramelli L, Bruschi G, Cosson V, Ratet P, de Larembergue H, Duc G, Piano E, Arcioni S (2008) An Italian functional genomic resource for Medicago truncatula BMC Res Notes 1:129. Rogers C, Wen J, Chen R, Oldroyd G Deletion-based reverse genetics in Medicago truncatula Plant Physiol. 2009 151(3):1077-86. Tadege M, Wang TL,Wen J, Ratet P, Mysore KS (2009) Mutagenesis and beyond! Tools for understanding legume biology. Plant Physiol. 151(3):978-84.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
