Subterranean Clover and Sulla as Valuable and Complementary Sources of Bioactive Compounds for Rainfed Mediterranean Farming Systems

Mediterranean pasture and forage legumes are important components of sustainable production systems. Subterranean clover and sulla represent key species having proven high agronomic value and traits for production and multiple services. Our research investigated the potential of the abovementioned species as a source of phenolic compounds and antioxidants for contributing to support their full exploitation in the fodder, animal welfare, and nutraceutical sectors. Antioxidant capacity, as well as the content of total phenolic compounds and individual phenolic compounds, was determined in subterranean clover and sulla shoots at the vegetative, flower bud, flowering, and seed ripening phenological stages. The antioxidant capacity and the phenolic content were affected significantly by harvest time. In subterranean clover, 10 individual phenolic compounds were detected, and isoflavones were the most abundant (3.19-18.27 mg·g-1 DM). Eleven phenolic compounds were identified in sulla shoots, and chlorogenic acid (0.76-3.43 mg·g-1 DM) and diosmin (3.64-4.94 mg·g-1 DM) were the most represented compounds at the vegetative and flower bud stage. On the basis of our findings, a complementary utilization of both legume species is suggested; this might ensure the exploitation of all phenolic compounds in view of the potential benefits for animal production and health.