Objectives. To analyze the presence of bovine ?-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of ?-LG immuno-like proteins (?-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. ?-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, ?-casein and ?-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine ?-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

Absence in human milk of bovine beta-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

G Giuffrida;
1997

Abstract

Objectives. To analyze the presence of bovine ?-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of ?-LG immuno-like proteins (?-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. ?-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, ?-casein and ?-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine ?-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.
1997
Beta lactoglobulin
affinity chromatography
animal
article
breast milk
cattle
chemistry
comparative study
diet
enzyme linked immunosorbent assay
female
human
laboratory diagnosis
methodology
milk
reproducibility
statistics
time
Animals
Cattle
Chromatography
Affinity
Diet
Enzyme-Linked Immunosorbent Assay
False Positive Reactions
Female
Humans
Lactoglobulins
Milk
Milk
Human
Reproducibility of Results
Time Factors
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/457847
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