Caff eic acid phenethyl ester (CAPE) is one of the most extensively investigated active components of propolis because of its numerous pharmacological activities, including anti-oxidant, anti-infl ammatory, anti-viral, anti-fungal, and regenerative properties. Two diff erent novel series of CAPE derivatives were designed, synthesized and their potential in bone regeneration was investigated. First, their eff ects on mesenchymal stem cell viability was evaluated: all the compounds showed no cytotoxicity and four of them appeared to stimulate cell proliferation at very low concentrations, in a manner similar to the reference compounds. Subsequently, Dental Pulp Stem Cells (DPSCs) were induced to osteogenic diff erentiation in presence of the most promising compounds (0-5 ?M) and cell proliferation, Alizarin Red Staining (ARS), alkalyn phosphatase (ALP) activity and BMP2, SP7 and DSPP gene expression were evaluated at 0, 7, 14, 21 and 28 days. DPSC exposed to all the compounds showed an increase in AR staining and in ALP activity at all the experimental times, and a modulation of the gene expression of the three well known markers of osteogenic diff erentiation. The adsorption of the compounds on a biomaterial synthesized for bone regeneration improved osteoblast adhesion and proliferation, as proved by preliminary results in confocal and stereoscopic microscopy. Concluding, our novel synthesized CAPE derivatives appeared to positively regulate osteogenic diff erentiation, showing a promising potential in regenerative medicine.
Novel CAPE derivatives enhance DPSC bone differentiation
Monica Rapino;
2022
Abstract
Caff eic acid phenethyl ester (CAPE) is one of the most extensively investigated active components of propolis because of its numerous pharmacological activities, including anti-oxidant, anti-infl ammatory, anti-viral, anti-fungal, and regenerative properties. Two diff erent novel series of CAPE derivatives were designed, synthesized and their potential in bone regeneration was investigated. First, their eff ects on mesenchymal stem cell viability was evaluated: all the compounds showed no cytotoxicity and four of them appeared to stimulate cell proliferation at very low concentrations, in a manner similar to the reference compounds. Subsequently, Dental Pulp Stem Cells (DPSCs) were induced to osteogenic diff erentiation in presence of the most promising compounds (0-5 ?M) and cell proliferation, Alizarin Red Staining (ARS), alkalyn phosphatase (ALP) activity and BMP2, SP7 and DSPP gene expression were evaluated at 0, 7, 14, 21 and 28 days. DPSC exposed to all the compounds showed an increase in AR staining and in ALP activity at all the experimental times, and a modulation of the gene expression of the three well known markers of osteogenic diff erentiation. The adsorption of the compounds on a biomaterial synthesized for bone regeneration improved osteoblast adhesion and proliferation, as proved by preliminary results in confocal and stereoscopic microscopy. Concluding, our novel synthesized CAPE derivatives appeared to positively regulate osteogenic diff erentiation, showing a promising potential in regenerative medicine.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
