Genome-wide survey of glutaredoxin gene family in four Solanaceae species and exploitation of duplicated CC-type following different environmental stimuli in tomato (Solanum lycopersicum)

Glutaredoxins (GRXs) are ubiquitous oxidoreductases involved in cell signaling, development and plant defenseresponse against oxidative stress. Despite this gene family has been characterized in several species, clues abouttheir evolution, diversification and function in Solanaceae are still in infancy. By exploring genomes of cultivatedtomato (Solanum lycopersicum), potato (S. tuberosum) and their wild relatives (S. pennellii, S. commersonii), a totalof 237 GRX genes were identified and grouped in CPYC-, CGFS-, GRL- and CC-types. Focusing on cultivatedtomato, we highlighted that a higher diversity within CC-type resulted from tandem duplication events, indi-cating that this latter might be the force driving GRXs evolution. The presence of a rich repertoire of cis-regu-latory elements suggested specialized functions of SlGRXs in plant development, hormonal and (a)biotic stressresponses, as also confirmed by their different expression patterns observed in various tissues and during fruitdevelopment through an in silico approach. qRT-PCR analysis revealed a divergent profile of tandem andsegmental duplicated CC-type members in tomato response to abiotic stresses, pathogenic or beneficial micro-organisms. This study reported the first insights on the GRX gene family in four Solanaceae species and provided acomprehensive and detailed analysis of their gene structure and role in tomato. The divergent profile of selectedduplicated CC-type members against various environmental constraints, highlighted candidate genes that will beused as a reference for functional analysis and genetic improvement not only in tomato but also in relatedspecies.