The detection of aflatoxin B in raw food materials represents a topic of great interest worldwide because of the huge health and economic impact of aflatoxin contamination. In this paper, we present an original approach to aflatoxin detection, using a portable instrument to acquire fluorescence images, among other spectral responses. The acquired images are processed by combining a color space conversion from the RGB scale to Y′CbCr, and a neural network approach to encode a vector of features. After a feature reduction using a Receiving Operating Curve method, two-class and three-class classification tasks of contaminated vs non-contaminated samples are accomplished. This procedure has been applied to artificially contaminated grained almond samples in the range of 0–320.2 ng/g, achieving an overall classification accuracy between 84.7% and 93.0%, depending on the number of classes. Thus, in this setting, we show that good classification performance can be achieved using only an image acquisition and analysis approach. The proposed procedure can represent a cheap, rapid, non-destructive yet sensitive method for the assessment of aflatoxin B contamination in food matrices, and its monitoring and tracing throughout the food chain.

Aflatoxins detection in almonds via fluorescence imaging and deep neural network approach

Bertani, Francesca Romana
Primo
Writing – Original Draft Preparation
;
Gambacorta, Lucia
Membro del Collaboration Group
;
De Ninno, Adele
Membro del Collaboration Group
;
Businaro, Luca
Membro del Collaboration Group
;
Solfrizzo, Michele
Membro del Collaboration Group
;
Gerardino, Annamaria
;
2024

Abstract

The detection of aflatoxin B in raw food materials represents a topic of great interest worldwide because of the huge health and economic impact of aflatoxin contamination. In this paper, we present an original approach to aflatoxin detection, using a portable instrument to acquire fluorescence images, among other spectral responses. The acquired images are processed by combining a color space conversion from the RGB scale to Y′CbCr, and a neural network approach to encode a vector of features. After a feature reduction using a Receiving Operating Curve method, two-class and three-class classification tasks of contaminated vs non-contaminated samples are accomplished. This procedure has been applied to artificially contaminated grained almond samples in the range of 0–320.2 ng/g, achieving an overall classification accuracy between 84.7% and 93.0%, depending on the number of classes. Thus, in this setting, we show that good classification performance can be achieved using only an image acquisition and analysis approach. The proposed procedure can represent a cheap, rapid, non-destructive yet sensitive method for the assessment of aflatoxin B contamination in food matrices, and its monitoring and tracing throughout the food chain.
2024
Istituto di fotonica e nanotecnologie - IFN
Istituto di Scienze delle Produzioni Alimentari - ISPA
Aflatoxin B rapid detection; Colour space conversion; Image analysis; Machine learning; Portable instrument
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/489761
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