The antimicrobial effect of titanium dioxide (TiO2) photocatalytic activity was reported by several studies. In recent years the TiO2 photocatalyst use as antimicrobial component of construction materials has been proposed. In this study a protocol for the investigation of the antibacterial activity of a UV irradiated TiO2-coated surface was developed. Glass Petri dishes with the bottom surface TiO2-coated were prepared. Bacterial suspensions of E. coli were tested in parallel on TiO2-coated Petri dishes UVA-irradiated vs. non-UVA-irradiated and on non-coated Petri dishes UVA-irradiated vs. non-UVA-irradiated. After the experimental treatment E.coli was recovered washing the Petri dishes and the percentage of surviving bacteria was calculated. Results highlight a decrease of the E.coli count in the TiO2-coated Petri dish when exposed to UVA radiation (3 h) in comparison with the non-irradiated one and with the negative control (non-TiO2 coated and non-irradiated) showing the antibacterial activity of the TiO2-coated Petri dish UVA irradiated. These preliminary data suggest the usefulness of this experimental protocol and design for the evaluation of the antibacterial activity of construction material surface treated with TiO2.
Evaluation of the antimicrobial activity of a surface-coated with TiO2
A Strini;
2007
Abstract
The antimicrobial effect of titanium dioxide (TiO2) photocatalytic activity was reported by several studies. In recent years the TiO2 photocatalyst use as antimicrobial component of construction materials has been proposed. In this study a protocol for the investigation of the antibacterial activity of a UV irradiated TiO2-coated surface was developed. Glass Petri dishes with the bottom surface TiO2-coated were prepared. Bacterial suspensions of E. coli were tested in parallel on TiO2-coated Petri dishes UVA-irradiated vs. non-UVA-irradiated and on non-coated Petri dishes UVA-irradiated vs. non-UVA-irradiated. After the experimental treatment E.coli was recovered washing the Petri dishes and the percentage of surviving bacteria was calculated. Results highlight a decrease of the E.coli count in the TiO2-coated Petri dish when exposed to UVA radiation (3 h) in comparison with the non-irradiated one and with the negative control (non-TiO2 coated and non-irradiated) showing the antibacterial activity of the TiO2-coated Petri dish UVA irradiated. These preliminary data suggest the usefulness of this experimental protocol and design for the evaluation of the antibacterial activity of construction material surface treated with TiO2.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
