This work presents the design and optimization of a miniaturizable, high-sensitivity system for real-time detection of adenosine triphosphate (ATP)-the primary energy carrier in biological systems-in water samples. Detection was based on the chemiluminescent ATP-luciferin driven reaction, monitored using a silicon photomultiplier (SiPM). Three different set-up configurations were investigated, focusing on the effects of reaction chamber size and total solution volume. The system achieved a minimum detectable ATP concentration of 0.05 nM, with a limit of detection (LoD) of 0.03 nM and a sensitivity of 11.9 A/M.
Development and Optimization of a Miniaturizable System for ATP Detection in Water Samples
Capuano G. E.;Screpis G. A.;Corso D.;Matera F.;Farina R.;Libertino S.
2025
Abstract
This work presents the design and optimization of a miniaturizable, high-sensitivity system for real-time detection of adenosine triphosphate (ATP)-the primary energy carrier in biological systems-in water samples. Detection was based on the chemiluminescent ATP-luciferin driven reaction, monitored using a silicon photomultiplier (SiPM). Three different set-up configurations were investigated, focusing on the effects of reaction chamber size and total solution volume. The system achieved a minimum detectable ATP concentration of 0.05 nM, with a limit of detection (LoD) of 0.03 nM and a sensitivity of 11.9 A/M.File in questo prodotto:
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