Viruses can affect grapevine yield and field performance, but the impact of infection on the quality and safety of final products has been scarcely investigated. This study reports the first analysis of agronomic performance, fruit texture and composition, and proteomic changes occurring in berries of virus-infected Vitis vinifera cv. Nebbiolo, grown in field conditions. 'Nebbiolo' clone 308 grapevines, carrying Grapevine leafrollassociated virus 1 (GLRaV-1), Grapevine virus A (GVA), and Rupestris stem pitting associated virus (RSPaV) in mixed infection, were compared to healthy vines obtained following heat treatment of the original infected mother plant. The phytosanitary status of each plant was assessed by reverse transcription-PCR, and GLRaV-1 and GVA concentration determined by quantitative reverse transcription-Real time PCR (qRTPCR). Mean loads of GLRaV-1 and GVA in the infected plants were 2.5 (SD=0.8) and 0.4 (SD=0.2) viral genomes/100 GAPDH transcript copies, respectively. The distribution of the viral loads was uniform among the infected plants. The comparison of field performance and fruit quality of healthy and infected plants revealed similar agronomical behaviour, with significant differences in titratable acidity, resveratrol content, and in some texture parameters. The proteomic analysis of skin and pulp from berries of healthy and virusinfected grapes evidenced significant differences for 12 pulp spots and 7 skin spots. Virus infection altered proteins involved in cell structure metabolism in the pulp, and proteins involved in the response to oxidative stress in the berry skin.
An integrated study on the effects of GLRaV-1, GVA and RSPaV on the field performance and berry quality of Vitis vinifera cv. Nebbiolo.
Pacifico D;Giribaldi M;Santini D;Mannini F;Caciagli P;Cavallarin L;Giuffrida MG;
2011
Abstract
Viruses can affect grapevine yield and field performance, but the impact of infection on the quality and safety of final products has been scarcely investigated. This study reports the first analysis of agronomic performance, fruit texture and composition, and proteomic changes occurring in berries of virus-infected Vitis vinifera cv. Nebbiolo, grown in field conditions. 'Nebbiolo' clone 308 grapevines, carrying Grapevine leafrollassociated virus 1 (GLRaV-1), Grapevine virus A (GVA), and Rupestris stem pitting associated virus (RSPaV) in mixed infection, were compared to healthy vines obtained following heat treatment of the original infected mother plant. The phytosanitary status of each plant was assessed by reverse transcription-PCR, and GLRaV-1 and GVA concentration determined by quantitative reverse transcription-Real time PCR (qRTPCR). Mean loads of GLRaV-1 and GVA in the infected plants were 2.5 (SD=0.8) and 0.4 (SD=0.2) viral genomes/100 GAPDH transcript copies, respectively. The distribution of the viral loads was uniform among the infected plants. The comparison of field performance and fruit quality of healthy and infected plants revealed similar agronomical behaviour, with significant differences in titratable acidity, resveratrol content, and in some texture parameters. The proteomic analysis of skin and pulp from berries of healthy and virusinfected grapes evidenced significant differences for 12 pulp spots and 7 skin spots. Virus infection altered proteins involved in cell structure metabolism in the pulp, and proteins involved in the response to oxidative stress in the berry skin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
