In this paper, a methodology for the analysis of subcellular morphology with chemical specificity, for images obtained by Stimulated Raman Scattering microscopy, is described. In order to detect microstructures inside the cells, a segmentation method based on a threshold algorithm and on a region growing process is proposed. Validation tests on images of polystyrene beads and of on adipocyte cells are reported, demonstrating the feasibility of our approach.
Features extraction for images from stimulated Raman scattering
N Brancati;M Indolfi;L Sirleto;M Frucci
2016
Abstract
In this paper, a methodology for the analysis of subcellular morphology with chemical specificity, for images obtained by Stimulated Raman Scattering microscopy, is described. In order to detect microstructures inside the cells, a segmentation method based on a threshold algorithm and on a region growing process is proposed. Validation tests on images of polystyrene beads and of on adipocyte cells are reported, demonstrating the feasibility of our approach.File in questo prodotto:
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