IL-17A is involved in the activation of oxidative stress and inflammation in nasal epithelial cells. Hyaluronan (HA) in its high molecular weight form (HMW-HA) shows anti inflammatory responses in contrast to low and medium molecular weight HA (LMW-HA and MMW-HA). The aim of this study was to investigate the pro- or anti-inflammatory biologic function of HA at different molecular weight in an in vitro model of nasal inflammation IL-17 mediated. We evaluated the ERK1/2 and I?B?phosphorylation, NF-?B signal pathway activation, ROS production, IL-8 and NOX-4 protein, and mRNA levels, in nasal epithelial cells RPMI 2650 stimulated with recombinant human (rh) IL-17A or PMA. Furthermore, the cells were treated with HMW-HA, MMW-HA, LMW-HA and U0126 (specific inhibitor of MEK1and MEK2 MAP kinase kinase). To confirm the specific action of HA, HMW-HA samples were degraded with Streptomyces hyaluronidase. Our results showed that rhIL-17A increased the ERK1/2, I?B? phosphorylation and NF-?B signal pathway activation, ROS production, IL-8 protein and mRNA levels, NOX-4 protein but not mRNA transcript. The addiction of HMW-HA or U0126 showed a significant downregulatory effect on inflammation due to the rhIL-17A stimulation in nasal epithelial cells. HMW-HA is able to downregulate the mechanism of uncontrolled oxidative stress and inflammation typical of upper airway diseases. Indeed, our results provided encouraging evidence to support possible beneficial effects of HMW-HA in the care of patients with altered function of nasal epithelial cells, supporting its potential ancillary role as coadjuvant of the classic anti-inflammatory treatment of the nose.
Effect of High, Medium and Low molecular weight hyaluronan on inflammation and oxidative stress in an in vitro model of Human Nasal Epithelial cells
Albano GD;Bonanno A;Di Sano C;Riccobono L;Gagliardo R;Montalbano AM;Anzalone G;Profita M
2016
Abstract
IL-17A is involved in the activation of oxidative stress and inflammation in nasal epithelial cells. Hyaluronan (HA) in its high molecular weight form (HMW-HA) shows anti inflammatory responses in contrast to low and medium molecular weight HA (LMW-HA and MMW-HA). The aim of this study was to investigate the pro- or anti-inflammatory biologic function of HA at different molecular weight in an in vitro model of nasal inflammation IL-17 mediated. We evaluated the ERK1/2 and I?B?phosphorylation, NF-?B signal pathway activation, ROS production, IL-8 and NOX-4 protein, and mRNA levels, in nasal epithelial cells RPMI 2650 stimulated with recombinant human (rh) IL-17A or PMA. Furthermore, the cells were treated with HMW-HA, MMW-HA, LMW-HA and U0126 (specific inhibitor of MEK1and MEK2 MAP kinase kinase). To confirm the specific action of HA, HMW-HA samples were degraded with Streptomyces hyaluronidase. Our results showed that rhIL-17A increased the ERK1/2, I?B? phosphorylation and NF-?B signal pathway activation, ROS production, IL-8 protein and mRNA levels, NOX-4 protein but not mRNA transcript. The addiction of HMW-HA or U0126 showed a significant downregulatory effect on inflammation due to the rhIL-17A stimulation in nasal epithelial cells. HMW-HA is able to downregulate the mechanism of uncontrolled oxidative stress and inflammation typical of upper airway diseases. Indeed, our results provided encouraging evidence to support possible beneficial effects of HMW-HA in the care of patients with altered function of nasal epithelial cells, supporting its potential ancillary role as coadjuvant of the classic anti-inflammatory treatment of the nose.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
