The kinetics of phosphopeptide release from gum fortified with a commercial casein hydrolysate inartificial and human saliva was studied. Using mass spectrometry techniques, thecaseinophosphopetide (CPP) composition of the commercial casein hydrolysate compared with a standard tryptic casein digest from whole raw cow milk was determined. In in vitro trials, theamount of CPPs released was always the same for each chewing time; in contrast, during in vivo chewing, a decrease of peptide levels was detected, from 10% after 5 min to 0.1% at 20 min, due to physiological swallowing. It is concluded that gums are a useful vehicle for CPP intake, owing to their functional role in bone health. However, their use is less satisfactory for prevention of dental caries, due to the brief contact with the enamel surface. Moreover, more calcium would need to be added to enrich the CPP fraction of the commercial hydrolysate.
Peptidomic study on in vitro and in vivo phosphopeptide release during the chewing of gum fortified with a commercial casein hydrolysate
Michele Di Stasio;Maria Grazia Volpe;
2018
Abstract
The kinetics of phosphopeptide release from gum fortified with a commercial casein hydrolysate inartificial and human saliva was studied. Using mass spectrometry techniques, thecaseinophosphopetide (CPP) composition of the commercial casein hydrolysate compared with a standard tryptic casein digest from whole raw cow milk was determined. In in vitro trials, theamount of CPPs released was always the same for each chewing time; in contrast, during in vivo chewing, a decrease of peptide levels was detected, from 10% after 5 min to 0.1% at 20 min, due to physiological swallowing. It is concluded that gums are a useful vehicle for CPP intake, owing to their functional role in bone health. However, their use is less satisfactory for prevention of dental caries, due to the brief contact with the enamel surface. Moreover, more calcium would need to be added to enrich the CPP fraction of the commercial hydrolysate.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.