Olive oil processing industries generate substantial quantities of phenolic-rich by-products, which could be valuable natural sources of antioxidants. This work is focused on the recovery and structural characterisation of antioxidant compounds from olive mill wastewater (OMWW), a polluting by-product of the olive oil production process. Phenolics were extracted from the waste material using a membrane technology coupled to a low-pressure gel filtration chromatography on a Sephadex LH-20. The LH-20 fraction was, in turn, characterized for its phenolic composition by HPLC-DAD-MS/MS analyses. Verbascoside, isoverbascoside, ?-hydroxyverbascoside, ?-hydroxyisoverbascoside, and various oxidized phenolics were identified. Uptake of verbascoside, purified from the LH-20 fraction, by HT-29 human colon carcinoma cell lines, an established model system for studying drug transport properties, was also assayed.
Recovery and Characterization of Verbascoside, Isoverbascoside and Their Derivatives from Olive Mill Waste Water
Cardinali A;Minervini F;D'Antuono I;Linsalata V;
2012
Abstract
Olive oil processing industries generate substantial quantities of phenolic-rich by-products, which could be valuable natural sources of antioxidants. This work is focused on the recovery and structural characterisation of antioxidant compounds from olive mill wastewater (OMWW), a polluting by-product of the olive oil production process. Phenolics were extracted from the waste material using a membrane technology coupled to a low-pressure gel filtration chromatography on a Sephadex LH-20. The LH-20 fraction was, in turn, characterized for its phenolic composition by HPLC-DAD-MS/MS analyses. Verbascoside, isoverbascoside, ?-hydroxyverbascoside, ?-hydroxyisoverbascoside, and various oxidized phenolics were identified. Uptake of verbascoside, purified from the LH-20 fraction, by HT-29 human colon carcinoma cell lines, an established model system for studying drug transport properties, was also assayed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
