Table olives are a commodity of great importance in the Mediterranean basin. One of the most important variety in Apulia (Southern Italy), is "Bella di Cerignola," that received the "Protected Denomination of Origin" (DOP) in 2000 by EU. This cultivar is mainly studied for its microbiological aspect1 but, actually, less is known regarding its specific phenolic compositions. Generally, olive drupes have high concentrations of phenolic compounds (? 1-2 % of fresh weight2), important for their antioxidant, anti-inflammatory and antitumoral properties, with beneficial effect on human health. The main objective of the present study was the characterization of phenolic compounds from "Bella di Cerignola" green, black and natural de-bittered marketable products. In addition, the assessment of bioaccessibility of these polyphenols was investigated by using in vitro gastro- intestinal digestion model3. The main polyphenols identified by HPLC-DAD and LC-HRMS were: hydroxytyrosol, tyrosol, caffeic acid, verbascoside, isoverbascoside, caffeoyl-6-secologanoside, comselogoside, luteolin. After in vitro digestion process, all total phenols identified were bioaccessible, apart luteolin that was absent in the digestive fraction. In particular, the bio-accessibilities were: 84.2% for hydroxytyrosol, 100% for tyrosol and caffeic acid, 47 % for verbascoside, 75% for isoverbascoside, 77% for caffeoyl-6-secologanoside, 81% for comselogoside. Regarding tyrosol, caffeic acid and isoverbascoside, their amount, in the chime fraction, was higher than no digested olive, probably for hydrolysis and isomerizations phenomena occurred in the gastro-intestinal conditions. These data are in good agreement with the results already published for the verbascoside biaccessibility4. In conclusion, the data obtained provide preliminary insight on the potential for bioavailability of the polyphenols present in a complex matrix such as "Bella di Cerignola" cultivar.
Biophenols of Table Olives: Assessment of Their Bioaccessibility After In Vitro Gastro-Intestinal Digestion
D'ANTUONO I;LINSALATA V;CIASCA B;LOGRIECO A F;CARDINALI A
2014
Abstract
Table olives are a commodity of great importance in the Mediterranean basin. One of the most important variety in Apulia (Southern Italy), is "Bella di Cerignola," that received the "Protected Denomination of Origin" (DOP) in 2000 by EU. This cultivar is mainly studied for its microbiological aspect1 but, actually, less is known regarding its specific phenolic compositions. Generally, olive drupes have high concentrations of phenolic compounds (? 1-2 % of fresh weight2), important for their antioxidant, anti-inflammatory and antitumoral properties, with beneficial effect on human health. The main objective of the present study was the characterization of phenolic compounds from "Bella di Cerignola" green, black and natural de-bittered marketable products. In addition, the assessment of bioaccessibility of these polyphenols was investigated by using in vitro gastro- intestinal digestion model3. The main polyphenols identified by HPLC-DAD and LC-HRMS were: hydroxytyrosol, tyrosol, caffeic acid, verbascoside, isoverbascoside, caffeoyl-6-secologanoside, comselogoside, luteolin. After in vitro digestion process, all total phenols identified were bioaccessible, apart luteolin that was absent in the digestive fraction. In particular, the bio-accessibilities were: 84.2% for hydroxytyrosol, 100% for tyrosol and caffeic acid, 47 % for verbascoside, 75% for isoverbascoside, 77% for caffeoyl-6-secologanoside, 81% for comselogoside. Regarding tyrosol, caffeic acid and isoverbascoside, their amount, in the chime fraction, was higher than no digested olive, probably for hydrolysis and isomerizations phenomena occurred in the gastro-intestinal conditions. These data are in good agreement with the results already published for the verbascoside biaccessibility4. In conclusion, the data obtained provide preliminary insight on the potential for bioavailability of the polyphenols present in a complex matrix such as "Bella di Cerignola" cultivar.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
