The aim of this study was to verify the activation details and products of human lymphomonocytes, stimulated by different -glucans, that is Euglena paramylon,MacroGard®, and lipopolysaccharide. We investigated the gene expression of inflammation-related cytokines and mediators, transactivation of relevant transcription factors, and phagocytosis role in cell-glucan interactions, by means of RT-PCR, immunocytochemistry, and colorimetric assay. Our results show that sonicated and alkalized paramylon upregulates pro-inflammatoryfactors (NO, TNF-?,IL-6,and COX-2) in lymphomonocytes. A clear demonstration of this upregulation is the increased transactivation of NF-kB visualized by immunofluorescence microscopy. Phagocytosis assay showed that internalization is not a mandatory step for signaling cascade to be triggered, since immune activity is not present in the lymphomonocytes that have internalized paramylon granules and particulate MacroGard®. Moreover, the response of Euglena ?-glucan-activated lymphomonocytes is much greater than that induced by commercially used ?-glucans such as MacroGard®. Our in vitro results indicate that linear fibrous Euglena ?-glucan, obtained by sonication and alkaline treatment can act as safe and effective coadjutant of the innate immune system response.
Euglena gracilis paramylon activates human lymphocytes by upregulating pro-inflammatory factors
Rossella Russo;Laura Barsanti;Vincenzo Longo;Laura Pucci;Paolo Gualtieri
2016
Abstract
The aim of this study was to verify the activation details and products of human lymphomonocytes, stimulated by different -glucans, that is Euglena paramylon,MacroGard®, and lipopolysaccharide. We investigated the gene expression of inflammation-related cytokines and mediators, transactivation of relevant transcription factors, and phagocytosis role in cell-glucan interactions, by means of RT-PCR, immunocytochemistry, and colorimetric assay. Our results show that sonicated and alkalized paramylon upregulates pro-inflammatoryfactors (NO, TNF-?,IL-6,and COX-2) in lymphomonocytes. A clear demonstration of this upregulation is the increased transactivation of NF-kB visualized by immunofluorescence microscopy. Phagocytosis assay showed that internalization is not a mandatory step for signaling cascade to be triggered, since immune activity is not present in the lymphomonocytes that have internalized paramylon granules and particulate MacroGard®. Moreover, the response of Euglena ?-glucan-activated lymphomonocytes is much greater than that induced by commercially used ?-glucans such as MacroGard®. Our in vitro results indicate that linear fibrous Euglena ?-glucan, obtained by sonication and alkaline treatment can act as safe and effective coadjutant of the innate immune system response.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.