Mycotoxins are toxic fungal metabolites that may contaminate several agricultural products, both in the field and during storage. Foodstuffs and feedstuffs contaminated by these natural contaminants may cause serious risks to human and animal health due to their toxic effects. For reliable surveillance programs is mandatory the availability of rapid and cost-effective methods for mycotoxin monitoring that should be validated according to harmonized guidelines. Performance evaluation according to internationally recognized validation programs ensures compliance with regulations and facilitates trade, giving the stakeholders more confidence to choose the proper assay with respect to the application. Development, validation and one-site testing of immunoassays for rapid mycotoxin detection is one of the priority tasks of the MycoKey project (http://www.mycokey.eu/), an EU multidisciplinary project coordinated by the Institute of Sciences of Food Production, National Research Council of Italy (ISPA-CNR), aimed to develop smart solutions to reduce the major occurring mycotoxins in economically important food and feed chains. A brief overview of recent activities carried out at ISPA-CNR on the development and validation of immunoassays for the rapid determination of mycotoxins in cereals and cereal-based products will be presented, with a focus on multi-mycotoxin assays for on-site application. In particular, performances, advantages and limitations of newly developed fluorescence polarization immunoassays and immunochromatographic assays (i.e. lateral flow devices or dipsticks) for the determination of the major mycotoxins occurring in cereals and cereal-based products, as well as the use of DNA aptamers as alternative novel biorecognition agents in biosensor applications for mycotoxins detection, will be presented.
ADVANCES IN SCREENING METHODS FOR MYCOTOXIN DETECTION IN CEREALS
Pascale M;Lippolis V;De Girolamo A;Logrieco AF
2017
Abstract
Mycotoxins are toxic fungal metabolites that may contaminate several agricultural products, both in the field and during storage. Foodstuffs and feedstuffs contaminated by these natural contaminants may cause serious risks to human and animal health due to their toxic effects. For reliable surveillance programs is mandatory the availability of rapid and cost-effective methods for mycotoxin monitoring that should be validated according to harmonized guidelines. Performance evaluation according to internationally recognized validation programs ensures compliance with regulations and facilitates trade, giving the stakeholders more confidence to choose the proper assay with respect to the application. Development, validation and one-site testing of immunoassays for rapid mycotoxin detection is one of the priority tasks of the MycoKey project (http://www.mycokey.eu/), an EU multidisciplinary project coordinated by the Institute of Sciences of Food Production, National Research Council of Italy (ISPA-CNR), aimed to develop smart solutions to reduce the major occurring mycotoxins in economically important food and feed chains. A brief overview of recent activities carried out at ISPA-CNR on the development and validation of immunoassays for the rapid determination of mycotoxins in cereals and cereal-based products will be presented, with a focus on multi-mycotoxin assays for on-site application. In particular, performances, advantages and limitations of newly developed fluorescence polarization immunoassays and immunochromatographic assays (i.e. lateral flow devices or dipsticks) for the determination of the major mycotoxins occurring in cereals and cereal-based products, as well as the use of DNA aptamers as alternative novel biorecognition agents in biosensor applications for mycotoxins detection, will be presented.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
