BACKGROUND: Pomegranate fruits are a rich source of polyphenols with numerous health-promoting effects. Pomegranate juices of five genotypes ("Mollar", "Kingdom", "Dente di Cavallo", and two old populations "Francofonte" and "Santa Tecla") were evaluated regarding anthocyanin and non-anthocyanin phenolic contents using UHPLC/Orbitrap-MS. Moreover total antioxidant activity (TAA) was evaluated using ABTS assay. RESULTS: 23 phenolic compounds were identified. Cyanidin-3,5-O-diglucoside and pelargonidin-3,5-O-diglucoside were the most representative anthocyanins in all genotypes; Santa Tecla population had the highest content of these anthocyanins, 97.64 mg/L and 40.29 mg/L respectively. In Francofonte population, ferulic acid hexoside was the most abundant compound (391.18 mg/L). Total antioxidant activity values ranged between 221.5 and 36.73 ?mol Trolox Equivalents/100 ml of juice. High Total Antioxidant Activity (TAA) value was recorded for Santa Tecla pomegranate population. CONCLUSION: In this paper, UHPLC/Orbitrap-MS approach was employed, for the first time, to identify the phenolic compound profiling in five pomegranate genotypes. Total antioxidant activity was analyzed using ABTS assay and the results showed a significant variability in nutraceutical potential of studied pomegranate genotypes. The inclusion of phenolic information in the Linear Discriminant Analysis (LDA) allowed reaching very good discriminations among genotypes.
Antioxidant activity and phenolic composition in Pomegranate (Punica granatum, L.) genotypes from south Italy by UHPLC/Orbitrap-MS approach
Rosaria Bognanni;Salvatore Scandurra;Maria Grazia Melilli
2018
Abstract
BACKGROUND: Pomegranate fruits are a rich source of polyphenols with numerous health-promoting effects. Pomegranate juices of five genotypes ("Mollar", "Kingdom", "Dente di Cavallo", and two old populations "Francofonte" and "Santa Tecla") were evaluated regarding anthocyanin and non-anthocyanin phenolic contents using UHPLC/Orbitrap-MS. Moreover total antioxidant activity (TAA) was evaluated using ABTS assay. RESULTS: 23 phenolic compounds were identified. Cyanidin-3,5-O-diglucoside and pelargonidin-3,5-O-diglucoside were the most representative anthocyanins in all genotypes; Santa Tecla population had the highest content of these anthocyanins, 97.64 mg/L and 40.29 mg/L respectively. In Francofonte population, ferulic acid hexoside was the most abundant compound (391.18 mg/L). Total antioxidant activity values ranged between 221.5 and 36.73 ?mol Trolox Equivalents/100 ml of juice. High Total Antioxidant Activity (TAA) value was recorded for Santa Tecla pomegranate population. CONCLUSION: In this paper, UHPLC/Orbitrap-MS approach was employed, for the first time, to identify the phenolic compound profiling in five pomegranate genotypes. Total antioxidant activity was analyzed using ABTS assay and the results showed a significant variability in nutraceutical potential of studied pomegranate genotypes. The inclusion of phenolic information in the Linear Discriminant Analysis (LDA) allowed reaching very good discriminations among genotypes.File | Dimensione | Formato | |
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Descrizione: Antioxidant activity and phenolic composition in Pomegranate (Punica granatum, L.) genotypes from south Italy by UHPLC/Orbitrap-MS approach
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