Determination of Histamine in Tuna Fish by a Rapid and Green DART‐TQ MS Method

This application note describes the development of a rapid and sustainable screening method for the detection of histamine (HIS) in food, representing a key biogenic amine and safety marker in tuna, based on the coupling between an ambient soft ionization source and a triple quadrupole mass spectrometer (DART–TQ‐MS/MS). The method requires minimal sample preparation and enables the targeted quantification of HIS through optimized MRM transitions (112.2 → 95.0 and 112.2 → 68.1 m/z). The DART–TQ‐MS/MS platform offers an efficient first‐line tool providing rapid preliminary control in complex food matrices before confirmatory analysis.