The fasibility of the production of anthocyanins by plant cell cultures has resulted in much research into the improvement of anthocyanin biosynthesis with the aim of producing an economically viable process for commercial application (Smith, 2000). The development of an efficient in vitro system for the production of anthocyanins requires an integrated approach which combins the effects of various enhancement strategies. Anthocyanins belong to the flavonoid family which has a well-studied pathway. The activity of chalcone synthase (CHS), the first enzyme in flavonoid biosynthesis, could be increased by elicitor treatment, wounding and light irradiation (Gleitz et al., 1991). Both biotic and abiotic elicitors enhance the synthesis of flavonoids in tissue culture systems. Jasmonic acid (JA) and its derivatives are believed to be involved in a signal transduction system which induces the production of defence compounds such as polyphenols or alkaloids. It has been reported that the combined action of JA and light improves the anthocyanin production in grape cell cultures (Zhang et al., 2002). Here we report the establishment of an anthocyanin producing calli derived from the leaves of sour cherry (Prunus cerasus L.) microplants. The prompting of anthocyanin production by light and JA and the modulation of the CHS protein synthesis following the first hours of treatments with the elicitors is also reported.
Characterization of sour cherry (Prunus cerasus L.) cell cultures in vitro producing anthocyanins
F Blando;I Nicoletti;
2004
Abstract
The fasibility of the production of anthocyanins by plant cell cultures has resulted in much research into the improvement of anthocyanin biosynthesis with the aim of producing an economically viable process for commercial application (Smith, 2000). The development of an efficient in vitro system for the production of anthocyanins requires an integrated approach which combins the effects of various enhancement strategies. Anthocyanins belong to the flavonoid family which has a well-studied pathway. The activity of chalcone synthase (CHS), the first enzyme in flavonoid biosynthesis, could be increased by elicitor treatment, wounding and light irradiation (Gleitz et al., 1991). Both biotic and abiotic elicitors enhance the synthesis of flavonoids in tissue culture systems. Jasmonic acid (JA) and its derivatives are believed to be involved in a signal transduction system which induces the production of defence compounds such as polyphenols or alkaloids. It has been reported that the combined action of JA and light improves the anthocyanin production in grape cell cultures (Zhang et al., 2002). Here we report the establishment of an anthocyanin producing calli derived from the leaves of sour cherry (Prunus cerasus L.) microplants. The prompting of anthocyanin production by light and JA and the modulation of the CHS protein synthesis following the first hours of treatments with the elicitors is also reported.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
